Consulta: creatorFacets:"Lesongeur, Francoise"
Registros recuperados: 28
Data/hora: 18/05/2024 14:56:51

Balneola alkaliphila sp nov, a marine bacterium isolated from the Mediterranean Sea
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Urios, L; Intertaglia, L; Lesongeur, Francoise; Lebaron, P.
A novel aerobic, Gram-negative bacterium, designated strain CM41_14b(T), was isolated from surface waters in the coastal north-western Mediterranean Sea. Cells were non-motile, straight rods, 2.6 mu m long and 0.7 mu m wide and formed pale-orange colonies on marine agar medium. The G + C content of the genomic DNA was 39 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence placed the strain within the genus Balneola (phylum Bacteroidetes). On the basis of 16S rRNA gene sequence comparisons, and physiological and biochemical characteristics, the isolate represents a novel species for which the name Balneola alkaliphila sp. nov. is proposed. The type strain is CM41_14(T) (=DSM 19538(T)=CIP 109603(T)=OOB 103(T)).
Tipo: Text
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-5292.pdf

Balneola vulgaris gen. nov., sp nov., a member of the phylum Bacteroidetes from the north-western Mediterranean Sea
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Urios, Laurent; Agogue, Hélène; Lesongeur, Francoise; Stackebrandt, Erko; Lebaron, Philippe.
A novel aerobic, Gram-negative bacterium, named 13IX/A01/164(T), was isolated from surface waters in the coastal north-western Mediterranean Sea. Cells were motile, straight rods, 2-5 mu m long and 0-2 mu m wide, and formed orange colonies on marine agar medium. The G+C content of the genomic DNA of strain 13IX/A01/164T was 42 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed the strain in the phylum Bacteroidetes within the family Crenotrichaceae. On the basis of 16S rRNA gene sequence comparison and physiological and biochemical characteristics, this isolate represents a novel species of a new genus, for which the name Balneola vulgaris gen. nov., sp. nov. is proposed. The type strain of Balneola vulgaris is 13IX/A01/164T (=DSM 17893T=CIP...
Tipo: Text
Palavras-chave: Mediterranean sea; Coastal waters; Microbiology; Physiology; Biochemistry; Gene sequence; Balneola vulgaris.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2126.pdf

Caloranaerobacter azorensis gen. nov., sp nov., an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Wery, Nathalie; Moricet, Jean-marc; Cueff, Valerie; Jean, Joelle; Pignet, Patricia; Lesongeur, Francoise; Cambon-bonavita, Marie-anne; Barbier, Georges.
A thermophilic, anaerobic, chemo-organotrophic bacterium, designated MV1087(T), was isolated from a deep-sea hydrothermal chimney sample collected from the Mid-Atlantic Ridge. The cells were straight, motile and stained Gram-negative. Growth was observed from 45 to 65 degreesC, with an optimum around 65 degreesC. No growth was observed at 40 or 70 degreesC. Growth was observed from pH 5.5 to 9.0 and the optimum pH was around 7. The salinity range for growth was 10-100 g sea salt l(-1) (corresponding to 6.5-65 g NaCl l(-1)) with an optimum at 30 g sea salt l(-1) (20 g NaCl l(-1)). Strain MV1087(T) was heterotrophic, able to ferment proteinaceous substrates, such as brain/heart infusion and gluten, and carbohydrates, such as glucose, xylan and starch. The...
Tipo: Text
Palavras-chave: Thermophile; Deep sea vent; Caloranaerobacter azorensis; Caloranaerobacter; Bacteria.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-586.pdf

Caminicella sporogenes gen. nov., sp nov., a novel thermophilic spore-forming bacterium isolated from an East-Pacific Rise hydrothermal vent
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Alain, Karine; Pignet, Patricia; Zbinden, M; Quillevere, M; Duchiron, F; Donval, Jean-pierre; Lesongeur, Francoise; Raguenes, Gerard; Crassous, Philippe; Querellou, Joel; Cambon-bonavita, Marie-anne.
A novel thermophilic, anaerobic, strictly chemoorganoheterotrophic bacterium, designated as AM1114(T), was isolated from a deep-sea hydrothermal vent sample from the East-Pacific Rise (EPR 13degrees N). The cells were long (3-10 mum) rods, motile with peritrichous flagella, and exhibited a Gram-negative cell wall ultrastructure. In the late stationary phase of growth, cells formed an ovoid, refractile, terminal endospore. They grew at 45-65 degreesC inclusive (optimum 55-60 degreesC; doubling time approx. 45 min), at pH 4.5-8.0 inclusive (optimum pH 7.5-8.0) and at sea salt concentrations of 20-60 g l(-1) inclusive (optimum 25-30 g l(-1)). Strain AM1114(T) was an obligately heterotrophic bacterium able to ferment a mixture of 20 amino acids, complex...
Tipo: Text
Palavras-chave: Caminicella sporogenes; Clostridiales; Thermophile; Deep sea hydrothermal vent.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-528.pdf

Comparative Study of Guaymas Basin Microbiomes: Cold Seeps vs. Hydrothermal Vents Sediments
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Cruaud, Perrine; Vigneron, Adrien; Pignet, Patricia; Caprais, Jean-claude; Lesongeur, Francoise; Toffin, Laurent; Godfroy, Anne; Cambon-bonavita, Marie-anne.
In the Guaymas Basin, the presence at a few tens of kilometers of cold seeps and hydrothermal vents coupled with comparable sedimentary settings and depths offer a unique opportunity to assess and compare the microbial community composition of these deep-sea ecosystems. The microbial diversity in sediments from three cold seep and two hydrothermal vent assemblages were investigated using high-throughput 16S rRNA-sequencing. Numerous bacterial and archaeal lineages were detected in both cold seep and hydrothermal vent sediments. Various potential organic matter degraders (e.g., Chloroflexi, Atribacteria, MBG-D) and methane and sulfur cycling related microorganisms (e.g., ANME and methanogenic lineages, sulfate-reducing lineages) were detected in both...
Tipo: Text
Palavras-chave: Microbial diversity; Hydrothermal vents; Cold seeps; Ribotypes; Methane; Extreme environments.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00415/52640/53496.pdf

Continuous enrichment culture and molecular monitoring to investigate the microbial diversity of thermophiles inhabiting deep-sea hydrothermal ecosystems
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Postec, Anne; Urios, Laurent; Lesongeur, Francoise; Ollivier, Bernard; Querellou, Joel; Godfroy, Anne.
The microflora developing during a continuous enrichment culture from a hydrothermal chimney sample was investigated by molecular methods. The culture was performed in a gas-lift bioreactor under anaerobic conditions, at 90 degrees C and pH 6.5, on a complex medium containing sulfur as the terminal electron acceptor. Archaeal and bacterial diversity was studied. Microorganisms affiliated with the genera Pyrococcus, Marinitoga, and Bacillus were detected through DGGE analysis of 16S rDNA. Additional sequences phylogenetically related to Thermococus and epsilon-Proteobacteria were detected by cloning and sequencing of 16S rDNA from two samples of the enrichment culture. In comparison, the sequences retrieved from cloning analysis from an enrichment culture...
Tipo: Text
Palavras-chave: Sequence alignment; Anaerobic digester; Thermotogales; Thermophile; Deep sea vent.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-364.pdf

Continuous enrichment cultures: insights into prokaryotic diversity and metabolic interactions in deep-sea vent chimneys
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Postec, Anne; Lesongeur, Francoise; Pignet, Patricia; Ollivier, B; Querellou, Joel; Godfroy, Anne.
The prokaryotic diversity of culturable thermophilic communities of deep-sea hydrothermal chimneys was analysed using a continuous enrichment culture performed in a gas-lift bioreactor, and compared to classical batch enrichment cultures in vials. Cultures were conducted at 60 degrees C and pH 6.5 using a complex medium containing carbohydrates, peptides and sulphur, and inoculated with a sample of a hydrothermal black chimney collected at the Rainbow field, Mid-Atlantic Ridge, at 2,275 m depth. To assess the relevance of both culture methods, bacterial and archaeal diversity was studied using cloning and sequencing, DGGE, and whole-cell hybridisation of 16S rRNA genes. Sequences of heterotrophic microorganisms belonging to the genera Marinitoga,...
Tipo: Text
Palavras-chave: Metabolic interactions; 16S rRNA gene; Thermophiles; Bioreactor; Continuous enrichment cultures; Deep sea hydrothermal vent; Microbial diversity.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3885.pdf

Desulfurobacterium crinifex sp.nov., a novel thermophilic, pinkish-streamer forming, chemolithoautotrophic bacterium isolated from a Juan de Fuca Ridge hydrothermal vent and amendment of the genus Desulfurobacterium
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Alain, Karine; Rolland, Sophie; Crassous, Philippe; Lesongeur, Francoise; Zbinden, Magali; Le Gall, Christian; Godfroy, Anne; Page, Antoine; Juniper, S Kim; Cambon-bonavita, Marie-anne; Duchiron, Francis; Querellou, Joel.
A novel thermophilic, chemolithoautotrophic T bacterium, designated as NE1206(T), was isolated from a Juan de Fuca Ridge hydrothermal vent sample (tubes of the annelid polychaete Paralvinella sulfincola attached to small pieces of hydrothermal chimney). The cells were rod-shaped (1.2-3.5x0.4-0.7 mum), occurring as single motile rods or forming macroscopic aggregates visible as pinkish to brownish streamers. The new isolate was anaerobic. It grew between 50 and 70 degreesC (optimum 60-65 degreesC doubling time approximately 1 h 15 min at 60 degreesC), between pH 5.0 and 7.5 (optimum pH around 6.0-6.5) and at sea salts concentrations between 20 and 40 g l(-1) (optimum 30 g l(-1)). Cells grew chemolithoautotrophically in an H-2/CO2 atmosphere (80/20, v/v; 200...
Tipo: Text
Palavras-chave: Thermophile; Nitrate reducing; Lithotroph; Desulfurobacterium crinifex; Deep sea hydrothermal vent; Cell networks.
Ano: 2003
URL: https://archimer.ifremer.fr/doc/2003/publication-663.pdf

Early diagenesis in the sediments of the Congo deep-sea fan dominated by massive terrigenous deposits: Part III – Sulfate- and methane- based microbial processes
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pastor, Lucie; Toffin, Laurent; Decker, Carole; Olu, Karine; Cathalot, Cecile; Lesongeur, Francoise; Caprais, Jean-claude; Bessette, Sandrine; Brandily, Christophe; Taillefert, M.; Rabouille, C.
Geochemical profiles (SO42-, H2S, CH4, δ13CH4) and phylogenetic diversity of Archaea and Bacteria from two oceanographic cruises dedicated to the lobes sediments of the Congo deep-sea fan are presented in this paper. In this area, organic-rich turbidites reach 5000 m and allow the establishment of patchy cold-seep-like habitats including microbial mats, reduced sediments, and vesicomyid bivalves assemblages. These bivalves live in endosymbiosis with sulfur-oxidizing bacteria and use sulfides to perform chemosynthesis. In these habitats, unlike classical abyssal sediments, anoxic processes are dominant. Total oxygen uptake fluxes and methane fluxes measured with benthic chambers are in the same range as those of active cold-seep environments, and oxygen is...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00376/48767/49164.pdf

Effect of variation of environmental conditions on the microbial communities of deep-sea vent chimneys, cultured in a bioreactor
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Byrne, Nathalie; Lesongeur, Francoise; Bienvenu, Nadege; Geslin, Claire; Alain, Karine; Prieur, Daniel; Godfroy, Anne.
Both cultivation and molecular techniques were used to investigate the microbial diversity and dynamic of a deep-sea vent chimney. The enrichment cultures performed in a gas-lift bioreactor were inoculated with a black smoker chimney sample collected on TAG site on the mid-Atlantic ridge. To mimic as close as possible environmental conditions, the cultures were performed in oligotrophic medium with nitrogen, hydrogen and carbon dioxide (N-2/H-2/CO2) gas sweeping. Also, the temperature was first settled at a temperature of 85A degrees C and colloidal sulphur was added. Then, the temperature was lowered to 60A degrees C and sulphur was omitted. Archaeal and bacterial diversity was studied in both culture and natural samples. Through 16S rRNA gene sequences...
Tipo: Text
Palavras-chave: 16S rRNA genes; Oligotrophic condition; Environmental condition; Bioreactor; Continuous enrichment culture; Microbial diversity; Deep sea hydrothermal vent.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6920.pdf

Export of deep-sea hydrothermal particles, indigenous thermophilic microorganisms and larvae to the surrounding Ocean
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lesongeur, Francoise; Briand, Patrick; Godfroy, Anne; Crassous, Philippe; Byrne, Nathalie; Khripounoff, Alexis.
To assess the production and the export of particulate and biological material in deep-sea hydrothermal vents, four moorings with sediment traps and current meters were deployed for 24 days in the Azores Triple Junction (ATJ) region. They were deployed along an axis starting at the base of a hydrothermal vent chimney and ending 1000 m away. The particles sampled at the base of the chimney were characterized by high concentrations in total sulfur (16%) and iron (4%). The particle composition changed drastically with distance from the vent: the sulfur concentration decreased with an increase in clay components. Thermophilic microorganisms were successfully enriched from all the particle samples. Cultured strains were closely related to hydrothermal species,...
Tipo: Text
Palavras-chave: Deep-sea hydrothermal vents; Particle flux; Hydrothermal microorganisms; Larvae; Regional index: North Atlantic; Mid-Atlantic Ridge; Azores Triple Junction.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00230/34111/33046.pdf

Haliea salexigens gen. nov., sp nov., a member of the Gammaproteobacteria from the Mediterranean Sea
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Urios, L; Intertaglia, L; Lesongeur, Francoise; Lebaron, P.
A novel aerobic, Gram-negative bacterium, designated 3X/A02/235(T), was isolated from the surface of coastal waters in the north-western Mediterranean Sea. Cells were motile, straight rods, 1.6 mu m long and 0.5 mu m wide, and formed cream colonies on marine agar medium. The G + C content of the genomic DNA was 61 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed the strain in the class Gammaproteobacteria and within the family Alteromonadaceae. On the basis of 16S rRNA gene sequence comparisons and physiological and biochemical characteristics, this isolate represents a novel species of a novel genus, for,which the name Haliea salexigens gen. nov., sp. nov. is proposed. The type strain of Haliea salexigens is 3X/A02/235(T) (=DSM 19537(T)...
Tipo: Text
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4193.pdf

Living (stained) deep-sea foraminifera from the Sea of Marmara: a preliminary study
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Fontanier, Christophe; Dissard, D.; Ruffine, Livio; Mamo, B.; Ponzevera, Emmanuel; Pelleter, Ewan; Baudin, F.; Roubi, Angelique; Cheron, Sandrine; Boissier, Audrey; Gayet, Nicolas; Bermell-fleury, S.; Pitel, M.; Guyader, Vivien; Lesongeur, Francoise; Savignac, F.
In this preliminary study, we investigate living (stained) foraminifera from the Sea of Marmara. We focus on the faunal composition and geochemical signatures (trace elements, carbon and oxygen stable isotopes) in foraminiferal tests at two deep-sea sites (329 and ~1240 m depth respectively). Documented by ROV observations and sampling, both study areas are heterogeneous (including bacterial mats and carbonate concretions), proximal to cold seeps and consist of dysoxic bottom water (O2&lt;20 µmol/L). The prevailing dysoxia at both study areas restricts foraminiferal diversity to very low values (S&lt;9, H’&lt;0.97). Stress-tolerant species Bolivina vadescens and Globobulimina affinis dominate living faunas at both sites. The highest foraminiferal standing...
Tipo: Text
Palavras-chave: Living (stained) benthic foraminifera; Sea of Marmara; Extreme ecosystems; Trace elements; Stable isotopes.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00416/52774/53646.pdf

Marinitoga camini gen, nov., sp nov., a rod-shaped bacterium belonging to the order Thermotogales, isolated from a deep-sea hydrothermal vent
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Wery, Nathalie; Lesongeur, Francoise; Pignet, Patricia; Derennes, Veronique; Cambon-bonavita, Marie-anne; Godfroy, Anne; Barbier, Georges.
A thermophilic, anaerobic, chemo-organotrophic sulfur-reducing bacterium, designated MV1075(T), was isolated from a deep-sea hydrothermal chimney sample collected on the Mid-Atlantic Ridge. Cells were rod-shaped with a sheath-like outer structure, motile with polar flagella and stained Gramnegative. They appeared singly, in pairs or in short chains. The temperature range for growth was 25-65 degreesC, with an optimum at 55 degreesC. Growth was observed from ph 5 to ph 9, and the optimum ph was around 7. The salinity range for growth was 15-70 g sea salt I-1 (corresponding to 10-45 g NaCl I-1), with an optimum at 30 g I-1 (20 g NaCl I-1). The isolate was able to grow on a broad spectrum of carbohydrates or complex proteinaceous substrates. Sulfur was not...
Tipo: Text
Palavras-chave: Marinitoga camini; Thermotogales; Thermophile; Deep sea hydrothermal vent.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-587.pdf

Marinitoga hydrogenitolerans sp nov., a novel member of the order Thermotogales isolated from a black smoker chimney on the Mid-Atlantic Ridge
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Postec, Anne; Le Breton, Claire; Fardeau, Marie-laure; Lesongeur, Francoise; Pignet, Patricia; Querellou, Joel; Ollivier, Bernard; Godfroy, Anne.
A novel, thermophilic, anaerobic bacterium that is able to tolerate hydrogen was isolated from a deep-sea hydrothermal chimney collected at the Rainbow field on the Mid-Atlantic Ridge. Cells were rod-shaped and surrounded by a sheath-like outer structure (toga); they were weakly motile by means of a polar flagellum. They appeared singly, in pairs or in short chains. They grew at 35-65 degrees C (optimum 60 degrees C), pH 4.5-8.5 (optimum pH 6.0) and 10-65 g sea salts l(-1) (optimum 30-40 g l(-1)). The isolate was organotrophic, and able to grow on various carbohydrates or complex proteinaceous substrates. Growth was not inhibited under 100% hydrogen or in the presence of 2% oxygen in the gas phase. The isolate reduces sulfur, although sulfur reduction is...
Tipo: Text
Palavras-chave: Anaerobic bacterium; Marinitoga hydrogenitolerans; Thermotogales; Thermophile; Deep sea vent.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-363.pdf

Melitea salexigens gen. nov., sp nov., a gammaproteobacterium from the Mediterranean Sea
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Urios, L; Agogue, H; Intertaglia, L; Lesongeur, Francoise; Lebaron, P.
A novel aerobic, Gram-negative bacterial strain, designated 51X/A01/131(T) T, was isolated from waters in the coastal north-western Mediterranean Sea. The cells were motile, straight rods, 1.6 mu m long and 0.5 pm wide, and formed cream colonies on marine 2216 agar. The G+C content of the genomic DNA was 57 mol %. Phylogenetic analysis of the 16S rRNA gene sequence placed the strain in the class Gammaproteobacteria. On the basis of the 16S rRNA gene sequence comparisons and physiological and biochemical characteristics, strain 51X/A01/13 1 T represents a novel genus and species, for which the name Melitea salexigens gen. nov., sp. nov. is proposed. The type strain of Melitea salexigens is 51X/A01/131(T) (=DSM 19753(T) =CIP 109757(T) =MOLA 225(T)).
Tipo: Text
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-5293.pdf

Microbial colonization of basaltic glasses in hydrothermal organic-rich sediments at Guaymas Basin
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Callac, Nolwenn; Rommevaux-jestin, Celine; Rouxel, Olivier; Lesongeur, Francoise; Liorzou, Celine; Bollinger, Claire; Ferrant, Anthony; Godfroy, Anne.
Oceanic basalts host diverse microbial communities with various metabolisms involved in C, N, S, and Fe biogeochemical cycles which may contribute to mineral and glass alteration processes at, and below the seafloor. In order to study the microbial colonization on basaltic glasses and their potential biotic/abiotic weathering products, two colonization modules called AISICS ("Autonomous in situ Instrumented Colonization System") were deployed in hydrothermal deep-sea sediments at the Guaymas Basin for 8 days and 22 days. Each AISICS module contained 18 colonizers (including sterile controls) filled with basaltic glasses of contrasting composition. Chemical analyses of ambient fluids sampled through the colonizers showed a greater contribution of...
Tipo: Text
Palavras-chave: Colonization module; Basalt alteration; Guaymas basin; Organic-rich sediment; Hydrothermal systems.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00181/29261/27642.pdf

Microbial communities associated with benthic faunal assemblages at cold seep sediments of the Sonora Margin, Guaymas Basin
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Cruaud, Perrine; Vigneron, Adrien; Pignet, Patricia; Caprais, Jean-claude; Lesongeur, Francoise; Toffin, Laurent; Godfroy, Anne; Cambon-bonavita, Marie-anne.
The Sonora Margin cold seeps present a seafloor mosaic pattern consisting of different faunal assemblages and microbial mats. To better understand if sedimentary microbial communities reflect this patchy distribution, all major habitats were investigated using four complementary approaches: 16S rRNA gene sequence 454 pyrosequencing, quantitative polymerase chain reaction, fluorescence in situ hybridization and geochemistry analyses. This study reveals that sediments populated by different surface assemblages show distinct porewater geochemistry features and are associated with distinct microbial communities. In the sediments underlying the microbial mat and the surrounding macrofauna, microbial communities were dominated by anaerobic methane oxidizers...
Tipo: Text
Palavras-chave: Microbial communities; Pyrosequencing; MBG-D; ANME; Faunal assemblages; Vesicomyidae; Siboglinidae; Microbial mat.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00288/39889/38434.pdf

Multidisciplinary investigation on cold seeps with vigorous gas emissions in the Sea of Marmara (MarsiteCruise): Strategy for site detection and sampling and first scientific outcome
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ruffine, Livio; Ondreas, Helene; Blanc-valleron, Marie-madeleine; Teichert, Barbara M. A.; Scalabrin, Carla; Rinnert, Emmanuel; Birot, Dominique; Croguennec, Claire; Ponzevera, Emmanuel; Pierre, Catherine; Donval, Jean-pierre; Alix, Anne-sophie; Germain, Yoan; Bignon, Laurent; Etoubleau, Joel; Caprais, Jean-claude; Knoery, Joel; Lesongeur, Francoise; Thomas, Bastien; Roubi, Angelique; Legoix, Ludovic Nicolas; Burnard, Pete; Chevalier, Nicolas; Lu, Hailong; Dupre, Stephanie; Fontanier, Christophe; Dissard, Delphine; Olgun, Nazli; Yang, Hailin; Strauss, Harald; Ozaksoy, Volkan; Perchoc, Jonathan; Podeur, Christian; Tarditi, Corinne; Ozbeki, Eyyup; Guyader, Vivien; Marty, Bernard; Madre, David G; Pitel-roudaut, Mathilde; Grall, Celine; Embriaco, Davide; Polonia, Mina; Gasperini, Lucas; Cagatay, M. Namik; Henry, Pierre; Geli, Louis.
MarsiteCruise was undertaken in October/November 2014 in the Sea of Marmara to gain detailed insight into the fate of fluids migrating within the sedimentary column and partially released into the water column. The overall objective of the project was to achieve a more global understanding of cold-seep dynamics in the context of a major active strike-slip fault. Five remotely operated vehicle (ROV) dives were performed at selected areas along the North Anatolian Fault and inherited faults. To efficiently detect, select and sample the gas seeps, we applied an original procedure. It combines sequentially (1) the acquisition of ship-borne multibeam acoustic data from the water column prior to each dive to detect gas emission sites and to design the tracks of...
Tipo: Text
Palavras-chave: Acoustic survey; Authigenic carbonates; Biogeochemistry; Chemical analyses; Cold seeps; Dissolved major elements; Fluid seepage; Geology; In situ Raman analysis; Methane; ROV dives.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00433/54434/55783.pdf

Nautilia abyssi sp nov., a thermophilic, chemolithoautotrophic, sulfur-reducing bacterium isolated from an East Pacific Rise hydrothermal vent
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Alain, Karine; Callac, Nolwenn; Guegan, Marianne; Lesongeur, Francoise; Crassous, Philippe; Cambon-bonavita, Marie-anne; Querellou, Joel; Prieur, Daniel.
A novel strictly anaerobic, thermophilic, sulfur-reducing bacterium, designated PH1209(T), was isolated from an East Pacific Rise hydrothermal vent (13 degrees N) sample and studied using a polyphasic taxonomic approach. Cells were Gram-negative, motile rods (approx. 1.60x0.40 mu m) with a single polar flagellum. Strain PH1209(T) grew at temperatures between 33 and 65 degrees C (optimum 60 degrees C), from pH 5.0 to 8.0 (optimum 6.0-6.5), and between 2 and 4% (w/v) NaCl (optimum 3%). Cells grew chemolithoautotrophically with H-2 as an energy source, So as an electron acceptor and CO2 as a carbon source. Strain PH1209(T) was also able to use peptone and yeast extract as carbon sources. The G+C content of the genomic DNA was 35 mol%. Phylogenetic: analyses...
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6585.pdf

Nisaea denitrificans gen. nov., sp nov and Nisaea nitritireducens sp nov., two novel members of the class Alphaproteobacteria from the Mediterranean Sea
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Urios, L; Michotey, V; Intertaglia, L; Lesongeur, Francoise; Lebaron, P.
Two novel Gram-negative bacteria, designated strains DR41_21(T) and DR41_18(T), were isolated from coastal, surface waters of the north-western Mediterranean Sea. The cells were motile, pleomorphic rods, 2.9 mu m long and 0.9 mu m wide and formed cream colonies on marine agar medium. The G + C content of the genomic DNA was 60 mol%. Phylogenetic analysis of 16S rRNA gene sequences positioned the isolates in the class Alphaproteobacteria within the family Rhodospirillaceae. The 16S rRNA gene sequence similarity of the two strains was 98.8% but DNA-DNA hybridization indicated only 55% relatedness. Strain DR41_21(T) was able to denitrify and possessed nirK and nosZ genes, unlike strain DR41_18(T), which possessed only nirK These isolates represent two novel...
Tipo: Text
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-5294.pdf

Presence and activity of anaerobic ammonium-oxidizing bacteria at deep-sea hydrothermal vents
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Byrne, Nathalie; Strous, Marc; Crepeau, Valentin; Kartal, Boran; Birrien, Jean-louis; Schmid, Markus; Lesongeur, Francoise; Schouten, Stefan; Jaeschke, Andrea; Jetten, Mike; Prieur, Daniel; Godfroy, Anne.
Recent studies indicate that ammonia is an important electron donor for the oxidation of fixed nitrogen, both in the marine water column and sediments. This process, known as anammox, has so far only been observed in a large range of temperature habitats. The present study investigated the role of anammox in hydrothermal settings. During three oceanographic expeditions to the Mid-Atlantic Ridge, hydrothermal samples were collected from five vent sites, at depths ranging from 750 to 3650m from cold to hot habitats. Evidence for the occurrence of anammox in these particular habitats was demonstrated by concurrent surveys, including the amplification of 16S rRNA gene sequences related to known anammox bacteria, ladderanes lipids analysis and measurement of a...
Tipo: Text
Palavras-chave: Hydrothermal vent; Ladderanes; 16S rRNA; Activity; Micro organisms; Anammox.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6921.pdf

Prokaryote Communities at Active Chimney and In‐Situ Colonization Devices after a Magmatic Degassing Event (37°N MAR, EMSO‐Azores Deep‐Sea Observatory)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rommevaux, Céline; Henri, Pauline; Degboe, Jefferson; Chavagnac, Valérie; Lesongeur, Francoise; Godfroy, Anne; Boulart, Cedric; Destrigneville, Christine; Castillo, Alain.
The mixing zone between high temperature hydrothermal fluids and seawater produces redox gradients, promoting the development of unique ecosystems based on chemotrophy. The structure of microbial communities depends on their environment, which can vary according to space and time. Hydrothermal circulation within the oceanic crust determines the chemical composition and flow of fluids, depending on underground events (earthquakes, volcanic episodes....), and impacts the development of microbial communities. This link between hydrothermal vent communities and deep geological events is the focus of the present study, the first of its kind for slow‐spreading ridge. In this study, we present a unique set of multidisciplinary data collected from 2008 to 2011 on...
Tipo: Text
Palavras-chave: Lucky Strike; Magmatic degassing; Microbial community structure; Multidisciplinary study.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00500/61174/64657.pdf

Relationship between the occurrence of filamentous bacteria on Bathymodiolus azoricus shell and the physiological and toxicological status of the vent mussel
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Martins, I.; Colaco, A.; Santos, Raphael; Lesongeur, Francoise; Godfroy, Anne; Sarradin, Pierre-marie; Cosson, R. P.
The edifice walls of the Eiffel Tower hydrothermal vent site (Mid-Atlantic Ridge, Lucky Strike vent field) are populated with dense communities of dual symbioses harboring vent mussel Bathymodiolus azoricus, some of which are covered by white filamentous mats belonging to sulfur-oxidizing bacteria. Mussels were collected in both the presence and absence of the filamentous bacteria. A sample of the filamentous bacteria was collected and water measurements of temperature, CH4 and H2S were recorded at the collection area. The whole soft tissues were analyzed for total lipid, carbohydrate and total protein. Metallothioneins and metals (Cu, Fe and Zn) levels were determined in the major organs. The results showed no significant physiological and toxicological...
Tipo: Text
Palavras-chave: Vent mussel; Metals; Metallothioneins; Filamentous bacteria; Eiffel Tower; Biomarkers.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6821.pdf

Relative abundance and diversity of bacterial methanotrophs at the oxic-anoxic interface of the Congo deep-sea fan
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Bessette, Sandrine; Moalic, Yann; Gautey, Sebastien; Lesongeur, Francoise; Godfroy, Anne; Toffin, Laurent.
Sitting at ~5 000 m water depth on the Congo-Angola margin and ~ 760 km offshore of the West African coast, the recent lobe complex of the Congo deep-sea fan receives large amounts of fluvial sediments (3-5% organic carbon). This organic-rich sedimentation area harbors habitats with chemosynthetic communities similar to those of cold seeps. In this study, we investigated relative abundance, diversity and distribution of aerobic methane-oxidizing bacteria (MOB) communities at the oxic-anoxic interface of sedimentary habitats by using fluorescence in situ hybridization and comparative sequence analysis of particulate mono-oxygenase (pmoA) genes. Our findings revealed that sedimentary habitats of the recent lobe complex hosted type I and type II MOB cells and...
Tipo: Text
Palavras-chave: Aerobic methane-oxidizing bacteria; PmoA; Organic-rich sediment; Methane seeps; Congo deep-sea fan.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00382/49327/49706.pdf

Structural Iron (II) of Basaltic Glass as an Energy Source for Zetaproteobacteria in an Abyssal Plain Environment, Off the Mid Atlantic Ridge
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Henri, Pauline A.; Rommevaux-jestin, Celine; Lesongeur, Francoise; Mumford, Adam; Emerson, David; Godfroy, Anne; Menez, Benedicte.
To explore the capability of basaltic glass to support the growth of chemosynthetic microorganisms, complementary in situ and in vitro colonization experiments were performed. Microbial colonizers containing synthetic tholeitic basaltic glasses, either enriched in reduced or oxidized iron, were deployed off-axis from the Mid Atlantic Ridge on surface sediments of the abyssal plain (35°N; 29°W). In situ microbial colonization was assessed by sequencing of the 16S rRNA gene and basaltic glass alteration was characterized using Scanning Electron Microscopy, micro-X-ray Absorption Near Edge Structure at the Fe-K-edge and Raman microspectroscopy. The colonized surface of the reduced basaltic glass was covered by a rind of alteration made of iron-oxides trapped...
Tipo: Text
Palavras-chave: Basaltic glass; Zetaproteobacteria; Bio-mediated alteration; Iron-oxidation; Abyssal plain.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00378/48894/49332.pdf

Thalassobaculum salexigens sp nov., a new member of the family Rhodospirillaceae from the NW Mediterranean Sea, and emended description of the genus Thalassobaculum
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Urios, Laurent; Michotey, Valerie; Intertaglia, Laurent; Lesongeur, Francoise; Lebaron, Philippe.
A novel Gram-negative bacteria, named CZ41_10a(T), was isolated from coastal surface waters of the north-western Mediterranean Sea. Cells were motile, pleomorphic rods, 1.6 mu m long and 0.7 mu m wide and formed cream colonies on marine agar medium. The G+C content of the genomic DNA was 65 mol%. Phylogenetic analysis of 16S rRNA gene sequences placed the new isolate in the genus Thalassobaculum, a member of the family Rhodospirillaceae, class Alphaproteobacteria. Unlike Thalassobaculum litoreum CL-GR58(T), its closest relative, strain CZ41_10a(T) was unable to grow anaerobically and did not exhibit nitrate reductase activity. On the basis of DNA-DNA hybridization, fatty acid content and physiological and biochemical characteristics, this isolate...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00001/11201/7846.pdf

The Congolobe project, a multidisciplinary study of Congo deep-sea fan lobe complex: Overview of methods, strategies, observations and sampling
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rabouille, C.; Olu, Karine; Baudin, F.; Khripounoff, Alexis; Dennielou, Bernard; Arnaud-haond, Sophie; Babonneau, Nathalie; Bayle, Christophe; Beckler, J.; Bessette, Sandrine; Bombled, B.; Bourgeois, S.; Brandily, Christophe; Caprais, Jean-claude; Cathalot, Cecile; Charlier, K.; Corvaisier, R.; Croguennec, Chantal; Cruaud, Perrine; Decker, Carole; Droz, L.; Gayet, Nicolas; Godfroy, Anne; Hourdez, S.; Le Bruchec, J.; Saout, Johan; Le Saout, Marie-helene; Lesongeur, Francoise; Martinez, P.; Mejanelle, L.; Michalopoulos, P.; Mouchel, Olivier; Noel, Philippe; Pastor, Lucie; Picot, M.; Pignet, Patricia; Pozzato, L.; Pruski, A. M.; Rabiller, Manuella; Raimonet, M.; Ragueneau, O.; Reyss, J. L.; Rodier, Philippe; Ruesch, Blandine; Ruffine, Livio; Savignac, F.; Senyarich, C.; Schnyder, J.; Sen, Arunima; Stetten, E.; Sun, Ming Yi; Taillefert, M.; Teixeira, S.; Tisnerat-laborde, N.; Toffin, Laurent; Tourolle, Julie; Toussaint, F.; Vetion, G.; Jouanneau, J. M.; Bez, M.
The presently active region of the Congo deep-sea fan (around 330 000 km2), called the terminal lobes or lobe complex, covers an area of 2500 km2 at 4700–5100 m water depth and 750–800 km offshore. It is a unique sedimentary area in the world ocean fed by a submarine canyon and a channel-levee system which presently deliver large amounts of organic carbon originating from the Congo River by turbidity currents. This particularity is due to the deep incision of the shelf by the Congo canyon, up to 30 km into the estuary, which funnels the Congo River sediments into the deep-sea. The connection between the river and the canyon is unique for major world rivers.  In 2011, two cruises (WACS leg 2 and Congolobe) were conducted to simultaneously investigate the...
Tipo: Text
Palavras-chave: Chemosynthetic habitats; Congo deep-sea fan; Fine sediment; Sedimentation rate; Seafloor morphology; Turbidite; Vesicomyidae.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00335/44580/44298.pdf